Confocal image of mouse lung infected with a non virulent strain of m.tuberculosis, stained with auramine-rhodamine (red/orange) and DAPI (blue)
This work was completed in collaboration with Allison St. Amand of the Pace Lab at University of Colorado at Boulder. The staining has been performed on a 10% neutral buffered formalin fixed, 4 micron paraffin section, the faint green staining is auto fluoresence due to the aldehyde fixation.
The mice were perfused 4% paraformaldehyde, tissues were embedded in agarose and 50 micron sections were cut on a…
This specimen was processed and embedded into paraffin without decalcification. Sections were cut at 5 microns on a standard microtome with a disposable blade.