Human EpiGingival™ organotypic culture construct from MatTek Corporation - Hematoxylin and Eosin, 200x

Normal human oral keratinocytes are differentiated into tissues with a non-cornified, buccal phenotype, or a cornified, gingival phenotype (as pictured).

The same cells are used to propagate both tissues.

The epithelium is cultured on a microporous membrane (Millipore Millicell™ CM cell culture inserts) on a plastic disk utilizing serum-free media.

This specimen was fixed in 4% paraformaldehyde.

The culture construct was removed from the well with an 8mm biopsy punch, processed between biopsy pads and bisected into two approximately equal halves prior to paraffin embedding.

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