Image Analysis

Densitometric, Morphometric & Stereometric Measurements

Premier Laboratory knows that accurate precise measurements begin with excellent sample preparation and staining. This holds true for something as seemingly simple as a routine H&E or as sophisticated as an immuno stain. Their carefully executed SOPs and personal attention to each and every slide ensures your satisfaction.

Advantages of high-throughput automated image analysis:

• Repetitive tasks are scored with both objectivity and consistency
• Measurements are precise with enhanced sensitivity
• Rare event detection can be performed
• Quantitative-based analysis – measure, count and classify
• Complex analytical questions can be answered
• Turnaround time is decreased
• Data is in generated in context of morphologic location – amount and localization
• Generates a permanent data record
• Quantitative rather than qualitative, so results are amenable to statistical analysis
• Analysis and documentation of subtle cellular changes not discernible to the human eye
• Execution of high volume analysis
• May be incorporated as pre-screen for further study development

Scalable resources give you the precise tools you need for your analysis. The extensive resources Premier Laboratory will put at your disposal include:

• Pathologists who are board-certified
• A dedicated staff of highly qualified experienced scientists, technologists and technicians
• The very latest in cutting-edge technology – IT, hardware and software

Typical measurements:

Morphometric measurements:
• Area
• Length/linear
• Diameter
• Count
• Percent fill
• Shape

Densitometric measurements:
• Staining intensity – nucleolar, nuclear, cytoplasmic or membranous

Stereometric measurements:
• Volume

Automated pattern recognition: Premier Laboratory’s standard and custom-designed algorithms can be fine tuned to classify images, based on any combination of morphometric and densitometric features to answer your complex investigative questions.

Study design: Premier Laboratory provides you with consultative and expert advice on a wide array of routine and esoteric research protocols. When combined these result in the design of the most cost effective, accelerated and efficacious study to meet your research goals. Their scalable product offering means you will achieve precisely the right combination of standard off-the-shelf and custom methodologies in the design of your research study. Validated regulated (GLP) and non-regulated investigative protocols are available.

Sample options:

• Quantification and analysis can be performed on your slides or on slides prepared for you by Premier Laboratory
• Histologic sections, TMAs, smears or monolayers
• Routine, special or immuno stained slides

Measuring the right area of interest is key to generating significant results. Options for selecting the area(s) for quantification include:

• Pathologist-based
• Automated pattern recognition driven
• Standard algorithm
• Custom algorithm

Report options: Your report structure is determined during study design, and is based on your need for validated regulated (GLP) and non-regulated investigative protocols. Technical reports on methods, both for staining and analysis validation are available. Complete statistical analysis packages may be incorporated.

Examples of image analysis applications:

• Percent or absolute number of cells stained
• Quantification of nuclear, cytoplasmic or membrane involvement
• Proliferation
• Apoptosis
• Tissue reaction to implanted devices
• Differential counts
• Determination of tumor sizes
• Follicle size
• Multi-stain quantification
• Histologic grading
• Blood vessel diameter
• Extent of infarcts
• Angiogenesis
• Muscle diameter
• Defect size and defect filled
• Nuclei location
• Bone mass
• Beta cell mass
• Measurement of mesenteric fat
• Atherosclerosis parameters